Dynamic covalent chemistry: Exploration of selective imine formation with synthetic and biological molecules and macromolecules
Messina - ITALIA
Strasburgo - FRANCIA
Messina
Artem Osypenko
Dipartimento:
Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali
Abstract
The current project explores the dynamic regioselective derivatization of synthetic and biological molecules for applications involving mapping the lysine distributions in monoclonal antibodies and tuning the activity of histories and retinal, e.g., for regulation of gene expression. The use of dynamic chemistry allows the thermodynamic control of the ligation reactions, which leads towards: i) the most stable products or their constitutional dynamic libraries (CDLs); ii) possibility for error-c.orrection; iii) responsive properties, namely the possibility to reverse the conjugation by changing the conditions (e.g., pH), opening the way to delivery applications.
Bioinorganic hybrid bacteriophage for specific antibacterial activity in the modulation of intestinal microbiota
Messina - ITALIA
Parigi - FRANCIA
Messina
Laura Maria De Plano
Dipartimento:
Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali
Abstract
Phage display technology hassuccessfully been established for discovery of epitope/mimotope peptides against pathogenic bacteria. The project will use engineered phages for the development of phage biohybrid complexes with nanostructured systems (i.e. nanoparticles) for new agents against bacteria diseases. The project will evaluate this approach against different bacterial species with a specific focus on establishing its efficiency, specificity and safety. The project includes (i) selection of M13 clones by phage-display technology with ability to selectively and specifically bind to molecular target of bacterial cell; ii) functionalization of the most responsive phage clones with antibacterial nano-materials; iii) in vitro bactericidal evaluation of the phage biohybrid system.